Kinetic Studies on the Arginine Kinase Reaction
نویسندگان
چکیده
منابع مشابه
Kinetic studies on the arginine kinase reaction.
A highly purified preparation of arginine kinase from the tail muscle of the crayfish, Panulirus longipes, has been used to investigate the mechanism of the reaction. The results of initial velocity, product inhibition, and isotope exchange at equilibrium studies are consistent with the reaction having a random mechanism in which all the steps are in rapid equilibrium except those concerned wit...
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or indirectly by a multienzyme system consisting of malic enzyme, malic dehydrogenase, and phosphoenolpyruvic carboxykinase (2, 3). However, the physiological significance of these two possible pathways has not been established. Lardy and Ziegler (4) demonstrated that the pyruvic kinase reaction is reversible in the presence of potassium and Meyerhof and Oesper (5) determined the apparent equil...
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The effect of temperature, pH, free [Mg(2+)], and ionic strength on the apparent equilibrium constant of arginine kinase (EC 2.7.3.3) was determined. At equilibrium, the apparent K' was defined as [see text] where each reactant represents the sum of all the ionic and metal complex species. The K' at pH 7.0, 1.0 mM free [Mg(2+)], and 0. 25 M ionic strength was 29.91 +/- 0.59, 33.44 +/- 0.46, 35....
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This work is a kinetic investigation of the reaction mechanism of malate dehydrogenase, prepared from washed mince of whole bovine heart by a variation on previous methods. The forward and reverse reactions catalyzed by this enzyme have been studied at pH 8.0 in the presence and in the absence of one product at a time, with the use of a recording fluorometer to measure changes in the concentrat...
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The mechanism of the hexokinase reaction has been the subject of numerous investigations in recent years (l-5). ijgren and Engstrijm reported the incorporation of Pa from labeled adenosine triphosphate (ATP) into the phosphoserine moiety of hexokinase in the absence of substrate glucose and concluded that an enzyme-phosphate intermediate participated in the reaction (2). Gamble and Najjar, howe...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1969
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)36405-0